Transcript quantitation in total yeast cellular RNA using kinetic PCR
نویسندگان
چکیده
منابع مشابه
Quantitation of HCV RNA using real-time PCR and fluorimetry.
Real-time PCR technology may provide an accurate and sensitive method to quantify hepatitis C virus (HCV) RNA. So far, studies have been carried out using the Taqman technology with the ABI Prism 7700 sequence detector. An alternative and simple real-time PCR assay is described with no probe requirement, based on the SYBR Green I dye and LightCycler fluorimeter. Amplicon synthesis was monitored...
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Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content. Here we describe the use of the compound o-phthaldialdehyde (OPA) to quantitate total protein u...
متن کاملQuantitation of residual WBCs in filtered blood components by high-throughput, real-time kinetic PCR.
BACKGROUND The effort to eliminate transfusion complications associated with WBCs has led to the widespread use of filters able to reduce WBC concentrations to <or=0.1 WBC per microL blood. This has necessitated sensitive QC methods to quantitate residual WBCs in filtered units. One fast, effective method is DNA amplification using real-time kinetic PCR (kPCR). STUDY DESIGN AND METHODS Two me...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.2.e2